Efficient in situ electroporation of mammalian cells grown on microporous membranes.

Tubular MFI Zeolite Membranes Made by In-Situ Crystallization

Polycrystalline MFI zeolite membranes were prepared on α-alumina tubular support by hydrothermal treatments at 413 K. The synthesized zeolite membranes were characterized by XRD, SEM and single gas permeation. The influence of hydrothermal treatment steps and the calcination time on the zeolite layer formation were investigated. MFI zeolite membrane prepared by four subsequent hydrothermal ...

Investigating the Effect of Various Oxidizing Agents on the Surface Functionalization of Microporous Polypropylene Membranes

Functional complementation by electroporation of human BACs into mammalian fibroblast cells.

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

DNA-Based Vaccine Is More Efficient than Non-Pathogenic Live Vaccine for the Prevention of HPV16 E7-Overexpressing Cancers

  Introduction: Vaccinology provides promising approaches for the control of various infectious diseases. Among different strategies, DNA vaccines offer attractive research opportunities for development of vaccines for induction of antigen-specific immunity owing to their stability, simplicity of delivery, safety and cost effectiveness. However, there is a need to increase their potency by the ...

High Efficient Transparent TiO2 Nanotube Dye-Sensitized Solar Cells: Adhesion of TiO2 Nanotube Membrane to FTO by Two Different Methods

In order to fabricate transparent TiO2 nanotube dye-sensitized solar cells, anodically growth nanotube membranes are detached from Ti substrate by a re-anodization method. The membranes are transferred on FTO glass by two different methods. At the first one, 100mM Ti-isopropoxide is used to make TiO2 nanoparticles for adhering TiO2 nanotube membranes to FTO and ...